Formation of Cell-sized Vesicles with Asymmetric Lipid Bilayer Using Pulsed Jet Flow
نویسندگان
چکیده
This paper describes the preparation of asymmetric cell-sized lipid vesicles from a planar lipid bilayer by using pulsed jet flow. First, the cell-sized vesicles (diameter of 5 μm in over 1 day) were stably formed using an improved double-well device wherein a separator with a small hole was mounted between the wells (Fig.1). Moreover, asymmetric vesicles of phosphatidylserine lipids were successfully formed on each leaflet using this device. The asymmetric cell-sized lipid vesicles will create new opportunities for artificial cell research model such as the interactions between lipid membranes and proteins.
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